Publication Date


Document Type


First Advisor

Hampel, Arnold E.

Degree Name

M.S. (Master of Science)

Legacy Department

Department of Biological Sciences


Hamsters; Ovaries; Cells--Morphology


Cell morphology profiles of several partial revertants of the temperature-sensitive Chinese Hamster Ovary cell line mutant, tsHl, which exhibits a defect in its leucyl-tRNA synthetase enzyme, were examined using light and scanning electron microscopy and were compared to the morphology profiles observed in wild-type and mutant cultures. Wild-type and tsHl morphology differences have been previously documented. Although the morphology of these cells changes as they progress through the cell cycle, wild-type cultures typically appear epithelial throughout interphase and display a randan distribution pattern, as opposed to tsHl cultures which tend to display a bipolar, spindle-shaped morphology. The relationship between these observed morphological changes and the altered leucyl-tRNA synthetase enzyme in idle mutant cells is unknown; however, morphological characterization of tsHl revertants suggests an apparent link between the enzyme defect and the observed bipolar morphology, since recovery of enzyme activity in the revertants is accompanied by a corresponding decrease in the appearance of the bipolar morphology type and an increase in the more spread out epithelial shape characteristic of wild-type cultures.


Includes bibliographical references.||Includes illustrations.


viii, 105 pages, 7 unnumbered pages




Northern Illinois University

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