Publication Date

1992

Document Type

Dissertation/Thesis

First Advisor

Yasui, Linda S.

Degree Name

M.S. (Master of Science)

Department

Department of Biological Sciences

LCSH

Ovaries||Cell lines

Abstract

The xrs-5 cell line, derived from the Chinese Hamster Ovary (CHO) K1 cell line, is defective in double strand break repair capabilities and is extremely radiosensitive, xrs-5 cells have a substantially lower survival rate compared to K1 cells. After six to eight weeks in culture, the xrs-5 cells revert to the K1 cell radiation sensitivity. Ultrastructural examination showed an increased separation between the inner and outer nuclear membranes. As the radiation sensitivity reverted, the nuclear membrane separation reverted to the K1 phenotype. In addition to membrane separation, cell and nuclear volume, nuclear to cytoplasmic ratio, and the surface area per unit volume of the cell were quantified for each cell line. The quantified morphological features were correlated with radiation sensitivity. No significant correlations were obtained except for the substantially higher degree of nuclear membrane separation in xrs-5 cells. It is concluded that the membrane separation of the nuclear envelope can be correlated with the radiation sensitivity of the xrs-5 cells, but it cannot be considered the sole cause of radiation sensitivity.

Comments

Includes bibliographical references (pages [60]-62)

Extent

x, 67 pages

Language

eng

Publisher

Northern Illinois University

Rights Statement

In Copyright

Rights Statement 2

NIU theses are protected by copyright. They may be viewed from Huskie Commons for any purpose, but reproduction or distribution in any format is prohibited without the written permission of the authors.

Media Type

Text

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