Bujarski, Jozef J.
M.S. (Master of Science)
Department of Biological Sciences
Cowpea; Virus diseases of plants; Viral genetics; Plant viruses
Full-length complementary DNA (cDNA) copies of genomic RNA1, RNA2, and RNA3 of cowpea chlorotic mottle virus (CCMV) S, D, and N strains were synthesized by using Vent DNA polymerase in polymerase chain reactions (PCR) and were cloned downstream of a 17 RNA polymerase promoter. Mixtures of capped in vitro transcripts from these clones produced typical CCMV symptoms when inoculated on soybean and cowpea plants. By using either gel separated viral RNA components or in vitro transcripts, pseudorecombinants S[sub 1]S[sub 2]D[sub 3], S[sub 1]S[sub 2]N[sub 3], D[sub 1]D[sub 2]S[sub 3] and N[sub 1]N[sub 2]S[sub 3] were constructed by exchanging RNA3 segments. The properties of these pseudorecombinants were tested by comparison of symptoms, dot-blot hybridization and reverse constructions to restore the parental virus strains. All other possible pseudorecombinants among D, N and S RNAs were prepared from in vitro transcripts and inoculated on a resistant soybean cultivar. The results from these experiments provided evidence for the involvement of CCMV RNA3 in necrotic lesion formation, systemic symptom development, and determination of virus concentration. Minor effects of CCMV RNA1 and RNA2 on systemic symptom formation and on virus concentration were also observed.
Shang, Hongzhong, "Mapping of host-related genetic information in cowpea chlorotic mottle virus" (1993). Graduate Research Theses & Dissertations. 3878.
v, 68 pages
Northern Illinois University
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