Publication Date

1993

Document Type

Dissertation/Thesis

First Advisor

Bujarski, Jozef J.

Degree Name

M.S. (Master of Science)

Legacy Department

Department of Biological Sciences

LCSH

Cowpea; Virus diseases of plants; Viral genetics; Plant viruses

Abstract

Full-length complementary DNA (cDNA) copies of genomic RNA1, RNA2, and RNA3 of cowpea chlorotic mottle virus (CCMV) S, D, and N strains were synthesized by using Vent DNA polymerase in polymerase chain reactions (PCR) and were cloned downstream of a 17 RNA polymerase promoter. Mixtures of capped in vitro transcripts from these clones produced typical CCMV symptoms when inoculated on soybean and cowpea plants. By using either gel separated viral RNA components or in vitro transcripts, pseudorecombinants S[sub 1]S[sub 2]D[sub 3], S[sub 1]S[sub 2]N[sub 3], D[sub 1]D[sub 2]S[sub 3] and N[sub 1]N[sub 2]S[sub 3] were constructed by exchanging RNA3 segments. The properties of these pseudorecombinants were tested by comparison of symptoms, dot-blot hybridization and reverse constructions to restore the parental virus strains. All other possible pseudorecombinants among D, N and S RNAs were prepared from in vitro transcripts and inoculated on a resistant soybean cultivar. The results from these experiments provided evidence for the involvement of CCMV RNA3 in necrotic lesion formation, systemic symptom development, and determination of virus concentration. Minor effects of CCMV RNA1 and RNA2 on systemic symptom formation and on virus concentration were also observed.

Comments

Includes bibliographical references (pages [64]-68)

Extent

v, 68 pages

Language

eng

Publisher

Northern Illinois University

Rights Statement

In Copyright

Rights Statement 2

NIU theses are protected by copyright. They may be viewed from Huskie Commons for any purpose, but reproduction or distribution in any format is prohibited without the written permission of the authors.

Media Type

Text

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