M.S. (Master of Science)
Department of Chemistry
Anesthetics--Physiological effects; Synaptosomes; Neural transmission
The acetylcholinesterase, (NaK)-ATPase and (Mg)- ATPase activities of rat brain synaptosomal membranes were measured as a function of increasing concentrations of local anesthetics. It was found that both the ouabain- sensitive (NaK)-ATPase and the ouabain-insensitive (Mg)- ATPase required about the same concentrations of local anesthetic for 50% inhibition, viz., lidocaine, 44 mM; tetracaine, 6 to 10 mM; dibucaine, 1.8 to 3.5 mM; and chlorpromazine, 0.22 to 0.25 mM. The logarithms of these concentratiohs are proportional to the logarithms of the respective partition coefficients. Inhibition of acetylcholinesterase required the following concentrations of anesthetic for 50% inhibition: procaine, 0.86 mM; lidocaine, 7.0 mM; tetracaine, 0.2 mM; dibucaine, 0.65 mM; and chlorpromazine, 0.21 mM. Inhibition of acetylcholinesterase by tetracaine and dibucaine was demonstrated to be competitive with substrate.
Mohd. Sidek, Hasidah, "Local anesthetic effects on synaptosomal acetylcholinesterase and adenosinetriphosphatase" (1983). Graduate Research Theses & Dissertations. 3816.
ix, 79 pages
Northern Illinois University
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