Norma K. Turk

Publication Date

1988

Document Type

Dissertation/Thesis

First Advisor

Conway, Thomas P.

Degree Name

M.S. (Master of Science)

Legacy Department

Department of Biological Sciences

LCSH

Immunoglobulins; Spleen--Surgery; Graft versus host reaction

Abstract

W. E. Briles, W. H. McGibbon, and M. R. Irwin, in 1950, identified the genes of the chicken MHC (B complex) which control histocompatibility antigens. Identification of these genes as part of the MHC complex was accomplished in part by means of graftversus- host (GVH) splenomegaly and inhibition of graft-versus-host splenomegaly (GVH-I) by specific anti-B alloantisera. In the course of their testing, several antisera were identified which did not inhibit splenomegaly as expected. These antisera were made by similar donor/recipient combinations and were of the same specificity (anti-B-F24) as antisera that did inhibit splenomegaly. Six alloantisera were separated into IgM and IgG fractions by gel filtration and tested for their ability to inhibit splenomegaly. Of the 6 alloantisera, 2 inhibited splenomegaly, 2 did not inhibit splenomegaly, and the other 2 were not previously tested. The alloantisera and the IgM and IgG fractions were tested for specific antibody titer by agglutination assay and by cellular radioimmunoassay. In those cases where the whole alloantisera was capable of inhibiting splenomegaly, the IgG fraction contained a significant proportion of the total inhibitory activity. The cellular radioimmunoassay titer of these IgG fractions was correspondingly high. In whole alloantisera which did not inhibit splenomegaly, the IgG fractions also did not inhibit splenomegaly and there was a correspondingly low cellular radioimmunoassay titer. The IgM fractions in all six alloantisera showed no significant inhibition, regardless of agglutination or cellular radioimmunoassay titer. In addition to the chicken alloantisera, a mouse monoclonal anti-B-L antibody was tested for ability to inhibit splenomegaly. Despite significant reactivity to lymphocytes, as shown by fluorescent immunoassay, there was no inhibition of splenomegaly.

Comments

Bibliography: pages [71]-79.

Extent

vi, 79 pages

Language

eng

Publisher

Northern Illinois University

Rights Statement

In Copyright

Rights Statement 2

NIU theses are protected by copyright. They may be viewed from Huskie Commons for any purpose, but reproduction or distribution in any format is prohibited without the written permission of the authors.

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Text

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