Starzyk, Marvin J.
M.S. (Master of Science)
Department of Biological Sciences
Staphylococci in water supplies is of great concern because of its potential pathogenicity. The abundance of these organisms on the human body allow them to be easily shed into bodies of water by swimmers, and also by sanitary sewage. At present, there are few methods for recovering these organisms from water. These various methods were evaluated as to quantitative recovery and degree of selectivity. A modified method is now being proposed which incorporates 2% egg yolk and 0.005% sodium azide into a mannitol salt agar base. This medium when used with a membrane filter procedure and incubated at 32°C for 48 hours recovers 85% of Staphylococcus aureus present. Environmental testing in this area indicates that this modified procedure will recover only Gram positive, catalase positive cocci. Ninety percent of these cocci are Staphylococcus as ascertained by the lysostaphin susceptibility test.
Stengren, Susan R., "Isolation of staphylococci from water" (1983). Graduate Research Theses & Dissertations. 3683.
viii, 59 pages
Northern Illinois University
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