Publication Date

1979

Document Type

Dissertation/Thesis

First Advisor

Hampel, Arnold E.

Degree Name

M.S. (Master of Science)

Department

Department of Chemistry

LCSH

Enzymes||Ribonucleic acid||Cytochemistry||Mutation (Biology)

Abstract

The mechanism of action of mammalian aminoacy1-tRNA synthetases has been aided by the development of mutants in these enzymes particularly in the Chinese hamster ovary (CHO) cell line. Comparison of one of these mutants, tsHl, with the wild type parent strain has previously revealed ah altered leucyl-tRNA synthetase in the mutant. In this report, another difference between these two cell lines is described. A factor, originally discovered in crude wild type polysomal preparations, has been observed to stimulate the activity of the tsHl leucyl-tRNA synthetase to a level greater than that of its wild type counterpart. This factor is heat labile, and further characterization reveals that it is associated with the wild type leucyl-tRNA synthetase complex. This factor may represent another level of control of protein synthesis in mammalian cells.

Comments

Includes bibliographical references.||Includes illustrations.

Extent

vii, 55 pages

Language

eng

Publisher

Northern Illinois University

Rights Statement

In Copyright

Rights Statement 2

NIU theses are protected by copyright. They may be viewed from Huskie Commons for any purpose, but reproduction or distribution in any format is prohibited without the written permission of the authors.

Media Type

Text

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