Publication Date

1995

Document Type

Dissertation/Thesis

First Advisor

Briles, W. Elwood

Degree Name

M.S. (Master of Science)

Department

Department of Biological Sciences

LCSH

Major histocompatibility complex||Graft versus host reaction||Chickens--Genetics||Genetic recombination

Abstract

The graft-versus-host (GVH) splenomegaly assay was used to identify the B- F/B-L regional segements among the twelve serologically identified major histocompatibility complex (MHC) recombinants. An additional objective was to detect GVH reactions that may be elicited by the three haplotypes of the Rfp-Y system. The three recombinants (ZfRl, B^, and 5^-10) that have been shown serologically to possess the B-F24 haplotype were tested against each other to determine if they were able to elicit a GVH reponse. The results indicate that these three recombinants possess the similar histocompatibility segment B-F24. Three other recombinants (B^, B^, and B& 11) that were shown serologically to possess the B-F21 histocompatibility region also gave compatible responses when they were tested against each other. The most recently identified recombinant, 5^12 was shown to contain the histocompatibility segment B-F8. The five recombinant haplotypes (B^, B^, B^, and B^) resulting from a recombination involving and B^ have been determined serologically to contain the histocompatibility segement B-F2. When these recombinants were tested against each other a single incompatible reaction was detected. The incompatible reaction occured when B^ containing cells were injected into embryos possessing the Z?R2 genotype. This incompatible reaction was weak, indicating that perhaps this mild reaction was due to some differences in non-5 histocompatibility antigens. Results of GVH splenomegaly reactions involving the Rfp-Y system demonstrated that in the scope of the testing performed, Rfp-Y does not influence GVH splenomegaly reactions. There were three weak incompatible responses after donor birds had been stimulated with foriegn Rfp-Y antigens. This mild GVH response is suggestive of possible Rfp-Y influence; however, a more plausable interpretation would be some non-B, non-F incompatibility.

Comments

Includes bibliographical references (pages [39]-47)

Extent

47 pages

Language

eng

Publisher

Northern Illinois University

Rights Statement

In Copyright

Rights Statement 2

NIU theses are protected by copyright. They may be viewed from Huskie Commons for any purpose, but reproduction or distribution in any format is prohibited without the written permission of the authors.

Media Type

Text

Share

COinS