Publication Date

1994

Document Type

Dissertation/Thesis

First Advisor

Johns, Mitrick A.

Degree Name

M.S. (Master of Science)

Department

Department of Biological Sciences

LCSH

Swine--Genetics||Swine--Breeding

Abstract

Random amplification of polymorphic DNA (RAPD) is a new technique (developed in 1990 by Williams et al. and Welsh and McClelland), which detects polymorphic DNA based on the amplification of undefined DNA segments with single primers of arbitrary nucleotide sequence. The fingerprint data created from this amplification process was used for analysis of genetic diversity between and within different lines of Sus Scrofa, maintained by DeKalb Swine Breeders stock. Seven purebred and five hybrid lines were used in the analysis. Data were analyzed using the Simple Matching method in conjunction with Principle Coordinate Analysis. The distances found between the lines define each group’s genetic diversity when compared to one another. The data revealed a definite distinction between groups. RAPD’s offer a quick, easy to use, cost-effective way to genetically separate groups of individuals and categorize them accordingly.

Comments

Includes bibliographical references (pages [78]-79)

Extent

93 pages

Language

eng

Publisher

Northern Illinois University

Rights Statement

In Copyright

Rights Statement 2

NIU theses are protected by copyright. They may be viewed from Huskie Commons for any purpose, but reproduction or distribution in any format is prohibited without the written permission of the authors.

Media Type

Text

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