Johns, Mitrick A.
M.S. (Master of Science)
Department of Biological Sciences
Random amplification of polymorphic DNA (RAPD) is a new technique (developed in 1990 by Williams et al. and Welsh and McClelland), which detects polymorphic DNA based on the amplification of undefined DNA segments with single primers of arbitrary nucleotide sequence. The fingerprint data created from this amplification process was used for analysis of genetic diversity between and within different lines of Sus Scrofa, maintained by DeKalb Swine Breeders stock. Seven purebred and five hybrid lines were used in the analysis. Data were analyzed using the Simple Matching method in conjunction with Principle Coordinate Analysis. The distances found between the lines define each group’s genetic diversity when compared to one another. The data revealed a definite distinction between groups. RAPD’s offer a quick, easy to use, cost-effective way to genetically separate groups of individuals and categorize them accordingly.
Morrow, Sasha L., "Genetic variation among swine lines from domestic swine breeders" (1994). Graduate Research Theses & Dissertations. 3264.
Northern Illinois University
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