Grosklags, James H.||Lynch, Darrel L.
M.S. (Master of Science)
Department of Biological Sciences
Fungi; Soil fungi; Stains and staining (Microscopy)
Single conidium and ascospore culture techniques were employed to demonstrate homothallism in several Emericellopsis species. Nuclei of all of the reported species of Emericellopsis (Eurotiaceae) were successfully demonstrated by staining with HCl-Giemsa following treatment in .002M 8-hydroxyquinoline and fixation in Carnoy’s with chloroform. The hyphal cells of all species were uninucleate with the exception of Emericellopsis synnematicola where binucleate cells were also observed. Those conidia and ascospores that were examined were found to be uninucleate. In some species, a high frequency of anastomosis and migrating nuclei suggested a means of establishing heterokaryons. Interphase nuclei, typically spherical, were extremely elongate near hyphal tips and nucleoli became extranucleate just prior to division. Mitotic figures were observed occasionally, and it was evident that the nuclei had a small number of chromosomes—perhaps three or four. Suggestions were made for improvement of staining and photographic techniques.
Benson, Bernard Wayne, "Differential staining of nuclei of emericellopsis" (1966). Graduate Research Theses & Dissertations. 2235.
vi, 77 pages
Northern Illinois University
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