Johns, Mitrick A.
M.S. (Master of Science)
Department of Biological Sciences
Genetics; Experimental; Cloning
A mouse MyoD cDNA probe was used to screen a Clonetec lambda phage porcine genomic library. Three distinct clones were isolated. Restriction fragments containing the genic regions were isolated from the clone that showed the strongest hybridization. These fragments were ligated into the vector plasmid pBS+. Further subcloning of these regions resulted in several smaller plasmids that were subjected to sequencing. The resultant data showed a high degree of homology with sheep, mouse, and human MyoD sequences. Sequencing has shown that Exon I has been subcloned along with nearly a kilobase of sequence at the 5’ end.
Smith, Glenn Sheridan, "Cloning and characterization of Exon I of the porcine MyoD gene" (1995). Graduate Research Theses & Dissertations. 1819.
Northern Illinois University
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