Publication Date

1966

Document Type

Dissertation/Thesis

First Advisor

Lynch, Darrel L.||Feyerherm, Harvey A.||Hess, Edwin A.

Degree Name

M.S. (Master of Science)

Department

Department of Biology

LCSH

Blood--Analysis||Leeches

Abstract

The proteins present in the blood of fourteen leeches, Haemopis marmoratis (Say), were characterized by various analytical methods. Electrophoretic techniques indicated that three proteins were present in the blood. Two dimensional paper chromatography of the free amino acid extraction indicated a minimum of free amino acids, with only aspartic acid evident. A two dimensional chromatogram of the protein amino acid content, after acid hydrolysis, indicated a constant number of fourteen amino acids forming an identical pattern in each chromatogram. This suggested the presence of the same three proteins in all fourteen blood samples tested. This also indicated that the blood is not a major storage site for either protein or amino acids. The presence of a dicarboxylic amino acid and lysine indicated some possible means for buffering mechanisms of the leech blood.

Comments

Includes bibliographical references.||Includes illustrations.

Extent

v, 24 pages, 8 unnumbered pages

Language

eng

Publisher

Northern Illinois University

Rights Statement

In Copyright

Rights Statement 2

NIU theses are protected by copyright. They may be viewed from Huskie Commons for any purpose, but reproduction or distribution in any format is prohibited without the written permission of the authors.

Media Type

Text

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