Lynch, Darrel L.||Feyerherm, Harvey A.||Hess, Edwin A.
M.S. (Master of Science)
Department of Biology
The proteins present in the blood of fourteen leeches, Haemopis marmoratis (Say), were characterized by various analytical methods. Electrophoretic techniques indicated that three proteins were present in the blood. Two dimensional paper chromatography of the free amino acid extraction indicated a minimum of free amino acids, with only aspartic acid evident. A two dimensional chromatogram of the protein amino acid content, after acid hydrolysis, indicated a constant number of fourteen amino acids forming an identical pattern in each chromatogram. This suggested the presence of the same three proteins in all fourteen blood samples tested. This also indicated that the blood is not a major storage site for either protein or amino acids. The presence of a dicarboxylic amino acid and lysine indicated some possible means for buffering mechanisms of the leech blood.
Fiordelisi, Rocco J., "Characterization of leech blood protein (Haemopis marmoratis, Say)" (1966). Graduate Research Theses & Dissertations. 1739.
v, 24 pages, 8 unnumbered pages
Northern Illinois University
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