Publication Date

1973

Document Type

Dissertation/Thesis

First Advisor

Briles, W. Elwood

Degree Name

M.S. (Master of Science)

Department

Department of Biological Sciences

LCSH

Poultry||Immunoglobulins||Antigens

Abstract

Matings were made between individual chickens derived from three breeds... White Leghorn, Rhode Island Red, New Hampshire... and a "mutant stock" to obtain families segregating for unique blood group antigens from non-Leghorn sources. The well established blood group antigens of the White Leghorn breed served as a reference for establishing identity or non-identity of antigens between and within the genetic groups. Reference reagents prepared by others in several inbred White Leghorn lines were used to identify tentatively the blood group antigens of the A, B, C, D, E, H, I, J, K, L and P blood group systems. Isoimmunizations were made within the families of this study in an effort to produce antibodies against unique blood group antigens proposed from an analysis of reference reagent agglutination cross-reactions. Absorptions were employed when two antigenic types appeared to differ only quantitatively in reactivity to a critical reagent. The specificities of the antibodies produced were determined by testing the antisera against erythrocytes of 62 previously typed Leghorns and 61 previously typed individuals resulting from backcrosses of New Hampshire, Rhode Island Red and mutant stock to the Leghorn. In the Rhode Island Red Breed, three blood group antigens were established. Two were unique (B₁₄ and B₁₅) and one (B₂₄) was shared with the New Hampshire Breed. Seven additional blood group antigens were suspected of being unique but could not be conclusively substantiated (C₇,C₈,C₉,I₈,I₉,I₁₀ and P₁₁), and sixteen antigens were proposed as being homologous to the respective Leghorn antigens (A₂,A₄,C₂,C₃,C₅,D₃,D₄,E₂,E₄,H₂,I₃,J₂,K₃,L₂,P₂ and P₅). In the New Hampshire breed three blood group antigens were established. Two were unique (B₂₃ and B₂₅) and one (B₂₄) was shared with the Rhode Island Red breed. Two additional antigens were tentatively proposed to be unique (E₁₇ and I₁₁) and twenty-one antigens were substantiated as homologs to the respective Leghorn types (A₂,A₃,A₄,C₂,C₃,C₄,D₃,E₁,E₂,E₅,E₇,H₂,I₃,I₅,I₆,J₁,J₂,K₂,K₃,L₁ and P₁₀). The mutant stock was found to have four unique blood group antigens (Bₘ₃,Bₘ₈,Bₘ₉ and Bₘ₁₁) as well as seventeen antigens homologous to the Leghorn breed (A₂,A₃,C₂,C₃,D₂,D₃,E₁,E₂,H₁,H₂,I₅,J₂,K₁,L₁,P₂,P₃ and P₅).

Comments

Includes bibliographical references.

Extent

61 pages

Language

eng

Publisher

Northern Illinois University

Rights Statement

In Copyright

Rights Statement 2

NIU theses are protected by copyright. They may be viewed from Huskie Commons for any purpose, but reproduction or distribution in any format is prohibited without the written permission of the authors.

Media Type

Text

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