Schjeide, Ole A.
M.S. (Master of Science)
Department of Biological Sciences
Bluntnose minnow; Fishes--Anatomy; Testis
Individuals belonging to the fish species Pimephales notatus were caught in the Kishwaukee river near Dekalb, Illinois. Testicular tissues were prepared for electron microscopy using a simultaneous glutaraldehyde and osmium tetroxide fixation procedure. Observations conducted on annulate lamellae and chromatoid bodies in the early spermatogenic series have revealed a number of commonly occurring features. These include: (a) the presence of annulate lamellae in close association with chromatoid bodies, (b) the existence of ahromatoid bands close to the nuclear envelope and continuous with chromatoid bodies, (c) the occurrence of chromatoid bands equidistantly spaced between adjacent annulate lamellae and between annulate lamellae and the nuclear envelope, (d) filamentous material connecting annulate lamellae to the chromatoid bodies (and bands) bridging a distance of approximately 90 nanometers and similar threads extending a like distance between chromatoid material and annuli of the nuclear pores, (e) mitochondria closely arranged about some chromatoid bodies, (f) Golgi complexes near the chromatoid masses with dense vesicles budding from Golgi cisternae and, (g) numerous vesicles or vesicular-like structures enclosed by or in contact with chromatoid bodies (and bands) displaying a periodicity similar to adjacent annulate lamellar pores. The observations are consistent with a nuclear origin of the chromatoid material which in turn controls the organization (formation) of annulate lamellae.
Graham, Galen Paul, "Annulate lamellae and chromatoid bodies in the testes of a cyprinid fish (Pimephales notatus)" (1972). Graduate Research Theses & Dissertations. 1481.
vi, 69 pages
Northern Illinois University
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