Frampton, Elon W.
M.S. (Master of Science)
Department of Biological Sciences
CsCl isopycnic density gradient centrifugation of lysates of UV- induced cultures of Escherichia coli strain 15 derivatives E. coli 15T- (thy) and E. coli 15 TAU (thy, arg, ura), yielded three phage peaks with buoyant densities of 1.509 g/cm³ (coliphage CP15A), 1.482 g/cm³ (CP15B), and 1.464 g/cm³ (CP15C). Agarose gel electrophoresis of EcoRI, HindiII, and BamHI endonuclease digests of the DNA from these three defective phage types yielded different digestion patterns, implying a unique genome for each phage type. Molecular weight estimates of genome size by agarose gel electrophoresis were 28 x 10⁶ daltons for CP15A, 26 x 10⁶ daltons for CP15B, and 25 x 10⁶ daltons for CP15C. Although the genomes are different, SDS-polyacrylamide ael electrophoresis revealed that the structural proteins of the tailed phages CP15B and CP15C were similar, whereas the CP15A proteins produced an entirely different pattern. The 65.8 x 10⁶ dalton Pl-like plasmid reported by Ikeda et al. (1970) was observed in agarose gels of two E. coli 15 derivatives.
Okazaki, Diane H., "Analysis of defective bacteriophages in Escherichia Coli strain 15 derivatives by restriction endonuclease digestion and SDS-polyacrylamide gel electrophoresis" (1983). Graduate Research Theses & Dissertations. 1450.
vi, 47 pages
Northern Illinois University
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