Author

Chi N. Tran

Publication Date

1997

Document Type

Dissertation/Thesis

First Advisor

Hudspeth, Michael E. S.

Degree Name

M.S. (Master of Science)

Department

Department of Biological Sciences

LCSH

Oomycetes--Phylogeny||Mitochondria

Abstract

The Phylum Oomycota, a group of "lower" or microfungi, have recently been placed in the newly erected Kingdom Straminopila. In this study, a mitochondrial gene has been used to infer a molecular phylogeny for representative genera of three major orders of oomycetes. The locus used (ndh9, nuo3, nad.9, or ND9) encodes subunit 9 of NADH:ubiquinone oxidoreductase (Complex I) of the respiratory electron transport chain Although normally nuclear-encoded in eukaryotes, ndh9 i mitochondrially encoded in plants and some lower eukaryotes, notably the Oomycota. The data set in this work consists of 401 nd.h.9 nucleotides generated by PCR from nine oomycete genera. Nucleotide data from the chlorophyte Prototheca wickerhamii were used as the outgroup in the analyses. Distance and character-state analysis by neighbor-joining (NJ), maximum parsimony (MP), and maximum likelihood (ML) analyses were used to infer an oomycete molecular phylogen^. The resulting phylogeny using ndh.9 is shown to be consistent with a previously generated cox2 phylogeny, and with a variety of both morphological and biochemical characters. The sole discrepancy with the cox2 phylogeny resides in the placement of Leptomitus of the Leptomitales.

Comments

Includes bibliographical references (pages [65]-71)

Extent

x, 73 pages

Language

eng

Publisher

Northern Illinois University

Rights Statement

In Copyright

Rights Statement 2

NIU theses are protected by copyright. They may be viewed from Huskie Commons for any purpose, but reproduction or distribution in any format is prohibited without the written permission of the authors.

Media Type

Text

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