Publication Date

1982

Document Type

Dissertation/Thesis

First Advisor

Vary, Patricia S.

Degree Name

M.S. (Master of Science)

Department

Department of Biological Sciences

LCSH

Plasmids||Bacteriocins||Bacillus megaterium

Abstract

Bacillus megaterium strains have been reported to produce bacteriocins known as megacins. Relatively large amounts of plasmid DNA have also been found. Since strain QM B1551 is currently of interest because its genome is being mapped by transduction, it was also tested for megacin production and the presence of plasmids. A megacin was found that was not UV inducible and was produced only on solid media by young cells. It was not comparable to megacin A, the best-characterized megacin, but was similar to megacin C. When tested for plasmids, strain QM B1551 had at least 11% of its total cellular* DNA recoverable in the form of covalently closed circular or plasmid DNA. This DNA contained several discrete size classes as determined by sucrose ultracentrifugation, agarose gel electrophoresis, and electron microscopy. The plasmid sizes were: 3.5, 5.3, 12, 31, 43, 70, and 109 million daltons (md). They were present in estimated copy numbers ranging from 1 for the 109 md plasmid to 135 for the smallest. This collection of plasmids was compared to that of other B. megaterium strains by simultaneous agarose gel electrophoresis and was found to be unique. The first determination of plasmids for strain KM, a megacin-sensitive strain, was also made.

Comments

Includes bibliographical references.||Includes illustrations.

Extent

vi, 79 pages

Language

eng

Publisher

Northern Illinois University

Rights Statement

In Copyright

Rights Statement 2

NIU theses are protected by copyright. They may be viewed from Huskie Commons for any purpose, but reproduction or distribution in any format is prohibited without the written permission of the authors.

Media Type

Text

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